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The Ligation Sequencing Kit contains a protocol for 1D c DNA sequencing. Nanopores are capable of analysing RNA as well as DNA, but in contrast to other sequencing technologies, nanopores are the only sequencing technology which can sequence RNA directly, rather than sequencing the products of reverse transcription and PCR reactions.Please read our cookie policy for more information and details on how to change your preferences. TV you love whenever you want Download the DIRECTV App to stream live and on-demand shows, up to 5 screens at a time.In Direct RNA Sequencing, only the native RNA passes through the nanopore sensor thus the read length reflects the length of the RNA molecules in the sample.
The Direct RNA Sequencing protocol contains an optional (but recommended) reverse transcription step and though the c DNA strand generated in this step is not sequenced, it significantly improves sequencing throughput.
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